Home > Patients & Visitors > Health Library > Antisperm Antibody Test
An antisperm antibody test looks for special proteins (antibodies) that fight against a man's sperm in blood, vaginal fluids, or semen. The test uses a sample of sperm and adds a substance that binds only to affected sperm.
Semen can cause an immune system response in either the man's or woman's body. The antibodies can damage or kill sperm. If a high number of sperm antibodies come into contact with a man's sperm, it may be hard for the sperm to fertilize an egg. The couple has a hard time becoming pregnant. This is called immunologic infertility.
A man can make sperm antibodies when his sperm come into contact with his immune system. This can happen when the testicles are injured or after surgeries (such as a biopsy or vasectomy) or after a prostate gland infection. The testicles normally keep the sperm away from the rest of the body and the immune system.
A woman can have an allergic reaction to her partner's semen and make sperm antibodies. This kind of immune response is not fully understood but may affect fertility. This is a rare cause of infertility.
The antisperm antibody test may be done if:
Talk to your doctor about any concerns you have regarding the need for the test, its risks, how it will be done, or what the results will mean. To help you understand the importance of this test, fill out the medical test information form( What is a PDF document? ).
For women, a blood sample is taken from a vein in the arm.
For men, a semen sample is collected after the blood and vaginal fluid samples are taken. You should not release your sperm (ejaculate) for 2 days before the test. It is important to not go longer than 5 days before the test without ejaculating.
The health professional taking a sample of your blood will:
A semen sample is collected by masturbation. You should urinate and then wash and rinse your hands and penis before collecting the semen in a sterile cup. You cannot use lubricants or condoms when collecting the sample. If you collect the semen sample at home, be sure to get it to the lab or clinic within 1 hour. Keep the sample at body temperature and out of direct sunlight. The sample cannot be collected by having sexual intercourse and then withdrawing when you ejaculate, because vaginal fluid may be mixed with the sperm.
The blood sample is taken from a vein in your arm. An elastic band is wrapped around your upper arm. It may feel tight. You may feel nothing at all from the needle, or you may feel a quick sting or pinch.
Collecting a semen sample does not cause any discomfort. If masturbation is against your religious beliefs, talk with your doctor.
There is very little chance of a problem from having a blood sample taken from a vein.
Usually there are no problems from collecting a semen sample.
An antisperm antibody test looks for special proteins (antibodies) that fight against a man's sperm in blood, vaginal fluids, or semen. The higher the level of antibody-affected sperm found in the semen, the lower the chance of the sperm fertilizing an egg.
Collecting a semen sample within 48 hours of ejaculating or after not ejaculating for longer than 5 days may affect the results of this test.
Other Works Consulted
Fischbach FT, Dunning MB III, eds. (2009). Manual of Laboratory and Diagnostic Tests, 8th ed. Philadelphia: Lippincott Williams and Wilkins.
Fritz MA, Speroff L (2011). Male infertility. In Clinical Gynecologic Endocrinology and Infertility, 8th ed., pp. 1249–1292. Philadelphia: Lippincott Williams and Wilkins.
Pagana KD, Pagana TJ (2010). Mosby's Manual of Diagnostic and Laboratory Tests, 4th ed. St. Louis: Mosby.
Current as of: May 29, 2019
Author: Healthwise StaffMedical Review: Sarah A. Marshall, MD - Family MedicineE. Gregory Thompson, MD - Internal MedicineAdam Husney, MD - Family MedicineKathleen Romito, MD - Family MedicineFemi Olatunbosun, MB, FRCSC, FACOG - Obstetrics and Gynecology, Reproductive Endocrinology
Current as of:
May 29, 2019
Medical Review:Sarah A. Marshall, MD - Family Medicine & E. Gregory Thompson, MD - Internal Medicine & Adam Husney, MD - Family Medicine & Kathleen Romito, MD - Family Medicine & Femi Olatunbosun, MB, FRCSC, FACOG - Obstetrics and Gynecology, Reproductive Endocrinology
To learn more about Healthwise, visit Healthwise.org.
© 1995-2019 Healthwise, Incorporated. Healthwise, Healthwise for every health decision, and the Healthwise logo are trademarks of Healthwise, Incorporated.
Our interactive Decision Points guide you through making key health decisions by combining medical information with your personal information.
You'll find Decision Points to help you answer questions about:
Get started learning more about your health!
Our Interactive Tools can help you make smart decisions for a healthier life. You'll find personal calculators and tools for health and fitness, lifestyle checkups and pregnancy.
Feeling under the weather?
Use our interactive symptom checker to evaluate your symptoms and determine appropriate action or treatment.